Background:
@EN Diuretic agents are widely used in the treatment of variable diseases. Although some diuretics may cause photosensitive reactions, this has been a relatively neglected area of investigation.
@ES Objective:
@EN The study was undertaken to examine whether or not the diuretic agents could cause phototoxicity with the photohemolysis test and with the Candida albicans test.
@ES Method: Photohemolysis test:
@EN Fresh human red blood cells (RBC) were washed and diluted to 200 fold of original volume with TCM buffer. Test compound suspensions were added to the diluted blood at the final concentration of 10-3M. Each sample was irradiated with 50 J/㎠of
UVA,
and control samples were incubated in water bath with light shielding. After additional incubation in a dark water bath for 30 minutes the samples were centrifuged. After adding Drabkin's reagent, measurement of absorbance of the supernatents by
spectrophotometer at 420, 540, and 550nm were performed. The degree of photohemolysis was determined by comparing the results with the control solution.
@ES Candida albicans test:
@EN Twenty microliter of each solution of various diuretics were applied to Sabouraud's dextrose agar plate in which Candida albicans had been diffusely applied previously. Four hours after the application, 80 J/㎠of UVA was irradiated. The
irraidated
plates andnonirradiated controls were incubated in a dark room for 48 hours, and were examined for clearzones arround the drugs as the mean of positive results for the phototoxic potential of the drugs.
@ES Results:
@EN The photohemolysis values of azosemide, spironolactone, tramterene, and xipamide at 420, 540, and 550nm were 70.57%/28.36%/31.61%, 83.65%/16.40%/15.21%, 75.66%/25.40%/28.17%, and 14.02%/9.11%/8.99% respectively, which exceeds the meaningful
positive
value of above 5%, With the Candida albida albicans test all test solution at concentration of 1% and 5% showed negative results.
@ES Conclusion:
@EN A phototoxic reaction may be triggered by azosemide, spironolactone, triamterene, and xipamide, and the possible mechanism concerned may be directed to cellular membranes. However with the negative results of known photosensitizers like
hydrochlorthiazide and furosemide, there may be other possibilities for the explanation of phototoxicity. Thus further studies may be necessary to investigate more details about the discrepancies in greates detail.
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